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human interleukin 3  (R&D Systems)


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    R&D Systems human interleukin 3
    Human Interleukin 3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+interleukin+3/Recombinant+Human+IL-3+Protein/pm41963354-281-22-26
    Average 96 stars, based on 260 article reviews
    human interleukin 3 - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Xeno-free Reprogramming of Peripheral Blood Mononuclear Erythroblasts on Laminin 521.
    Article Snippet: .. Dissolve 10 μg recombinant human Interleukin-3 (carrier-free; R&D Systems, 203-GMP-010) in 100μl DPBS −/− to make a 100 μg/ml (2,000x) stock. ..

    Article Title: Supramolecular assembly of GSK3α as a cellular response to amino acid starvation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Asparaginase (pegaspargase) Shire Pharmaceuticals Oncaspar Trypan blue Invitrogen Cat#T10282 Human FLT3 R&D systems Cat#398-FK Human interleukin-7 R&D systems Cat#206-IL-010 Human stem cell factor R&D systems Cat#255-SC Puromycin Thermo Fisher Cat#A1113803 Neomycin Carl Roth Cat #CP11.2 Blasticidin Thermo Fisher Cat#R21001 Polybrene Merck Millipore Cat#TR-1003-G Lipofectamine 2000 Invitrogen Cat#11668030 Dexamethasone Sigma-Aldrich Cat#D4902 Vincristine Selleckchem Cat#S1241 6-Mercaptopurine Abcam Cat#ab142389 Rapamycin Selleckchem Cat#S1039 BRD5648 (Wagner et al., 2018) N/A BRD0705 (Wagner et al., 2018) N/A BRD3731 (Wagner et al., 2018) N/A HSP70 inhibitors B (Kang et al., 2014; Taldone et al., 2014). .. CD34+ progenitors were cultured in RPMI (Thermo Fisher Scientific) supplemented with 20% FBS and recombinant human interleukin-3 (R&D systems, Minneapolis, MN), recombinant human interleukin-6 (R&D systems, Minneapolis, MN) and recombinant human stem cell factor (R&D systems, Minneapolis, MN) to a final concentration of 50 ng/ml each prior to intended use. .. N/A Bortezomib Sigma-Aldrich Cat#5043.14 Chloroquine Sigma-Aldrich Cat#PHR1258 Geldanamycin Sigma-Aldrich Cat#G3381 RIPA buffer Merck Millipore Cat#20-188 Complete Protease Inhibitor Roche Cat#05892970001 PhosSTOP phosphatase inhibitor Roche Cat#4906845001 Laemmli sample buffer Bio-Rad Cat#161-0737 b-mercaptoethanol Sigma-Aldrich Cat#M6250 4-12% bis-tris polyacrylamide gel BioRad Cat#4568093 PVDF membrane Carl Roth Cat# T830.1 BSA Carl Roth Cat#8076.4 Sure Block LuBio Science Cat#SB232010 ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat# P36962 A/G agarose beads Thermo Fisher Cat#20423 Trichloroacetic acid Sigma-Aldrich Cat#T6399 Acetone J.T.

    Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome.
    Article Snippet: On days 0, 2, and 4 of differentiation, cells were cultured in SF- DIFF medium and further supplemented with the following cytokines and chemicals: day 0: 0.5 mM L- ascorbic acid (Sigma- Aldrich), 0.45 mM monothioglycerol (Fujifilm Wako Chemicals), 5 ng/mL recombinant human bone morphogenic protein- 4 (rhBMP- 4, R&D Systems, Minneapolis, MN, USA), 5 ng/mL recombinant human fibroblast growth factor 2 (rhFGF2, R&D Systems); day 2: 0.5 mM L- ascorbic acid, 0.45 mM monothioglycerol, 2 ng/ mL rhBMP- 4, 5 ng/mL rhFGF2, 5 ng/mL recombinant human vascular endothelial growth factor (rhVEGF, R&D Systems), and 2 ng/mL recombinant human/mouse/rat activin A (rhactivin A, R&D Systems); day 4: 0.5 mM L- ascorbic acid, 0.45 mM monothioglycerol, 25 μg/mL gentamicin (FUJIFILM Wako Pure Chemical Corporation), 5 ng/mL rhVEGF and 100 ng/ mL recombinant human colony stimulating factor 1 (rhCSF- 1, R&D Systems). .. On day 6, the culture medium was switched to StemPro- 34 medium (Thermo Fisher Scientific) containing 0.5 mM L- ascorbic acid, 0.45 mM monothioglycerol, penicillin–streptomycin (1×), 150 μg/mL transferrin from human serum (Roche, Basel, Switzerland), 2.0 mM glutamine, 50 ng/ mL rhCSF- 1, 100 ng/mL recombinant human stem cell factor (rhSCF, Nacalai Tesque), 10 ng/mL recombinant human interleukin- 3 (rhIL- 3, R&D Systems). mESCs or embryonic bodies were dissociated in TrypLE (1×, Thermo Fisher Scientific) for 4 min and collected by centrifugation at 200 × g for 5 min using a table- top centrifuge (Model2420, KUBOTA, Tokyo, Japan). .. For terminal differentiation towards primitive macrophages, collected cells were seeded with SF- DIFF (days 0, 2, and 4) or StemPro medium (day 6) into non- coated dishes (days 0, 2, and 6) and poly (2- hydroxyethyl methacrylate) (HEMA, SigmaAldrich)- coated plates (day 4) at 2.0–5.0 × 105 cells/mL and incubated under 5% CO2 at 37°C.

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome
    Article Snippet: On days 0, 2, and 4 of differentiation, cells were cultured in SF‐DIFF medium and further supplemented with the following cytokines and chemicals: day 0: 0.5 mM L‐ascorbic acid (Sigma‐Aldrich), 0.45 mM monothioglycerol (Fujifilm Wako Chemicals), 5 ng/mL recombinant human bone morphogenic protein‐4 (rhBMP‐4, R&D Systems, Minneapolis, MN, USA), 5 ng/mL recombinant human fibroblast growth factor 2 (rhFGF2, R&D Systems); day 2: 0.5 mM L‐ascorbic acid, 0.45 mM monothioglycerol, 2 ng/mL rhBMP‐4, 5 ng/mL rhFGF2, 5 ng/mL recombinant human vascular endothelial growth factor (rhVEGF, R&D Systems), and 2 ng/mL recombinant human/mouse/rat activin A (rhactivin A, R&D Systems); day 4: 0.5 mM L‐ascorbic acid, 0.45 mM monothioglycerol, 25 μg/mL gentamicin (FUJIFILM Wako Pure Chemical Corporation), 5 ng/mL rhVEGF and 100 ng/mL recombinant human colony stimulating factor 1 (rhCSF‐1, R&D Systems). .. On day 6, the culture medium was switched to StemPro‐34 medium (Thermo Fisher Scientific) containing 0.5 mM L‐ascorbic acid, 0.45 mM monothioglycerol, penicillin–streptomycin (1×), 150 μg/mL transferrin from human serum (Roche, Basel, Switzerland), 2.0 mM glutamine, 50 ng/mL rhCSF‐1, 100 ng/mL recombinant human stem cell factor (rhSCF, Nacalai Tesque), 10 ng/mL recombinant human interleukin‐3 (rhIL‐3, R&D Systems). mESCs or embryonic bodies were dissociated in TrypLE (1×, Thermo Fisher Scientific) for 4 min and collected by centrifugation at 200 × g for 5 min using a table‐top centrifuge (Model2420, KUBOTA, Tokyo, Japan). .. For terminal differentiation towards primitive macrophages, collected cells were seeded with SF‐DIFF (days 0, 2, and 4) or StemPro medium (day 6) into non‐coated dishes (days 0, 2, and 6) and poly (2‐hydroxyethyl methacrylate) (HEMA, Sigma‐Aldrich)‐coated plates (day 4) at 2.0–5.0 × 10 5 cells/mL and incubated under 5% CO 2 at 37°C.

    Cell Culture:

    Article Title: Supramolecular assembly of GSK3α as a cellular response to amino acid starvation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Asparaginase (pegaspargase) Shire Pharmaceuticals Oncaspar Trypan blue Invitrogen Cat#T10282 Human FLT3 R&D systems Cat#398-FK Human interleukin-7 R&D systems Cat#206-IL-010 Human stem cell factor R&D systems Cat#255-SC Puromycin Thermo Fisher Cat#A1113803 Neomycin Carl Roth Cat #CP11.2 Blasticidin Thermo Fisher Cat#R21001 Polybrene Merck Millipore Cat#TR-1003-G Lipofectamine 2000 Invitrogen Cat#11668030 Dexamethasone Sigma-Aldrich Cat#D4902 Vincristine Selleckchem Cat#S1241 6-Mercaptopurine Abcam Cat#ab142389 Rapamycin Selleckchem Cat#S1039 BRD5648 (Wagner et al., 2018) N/A BRD0705 (Wagner et al., 2018) N/A BRD3731 (Wagner et al., 2018) N/A HSP70 inhibitors B (Kang et al., 2014; Taldone et al., 2014). .. CD34+ progenitors were cultured in RPMI (Thermo Fisher Scientific) supplemented with 20% FBS and recombinant human interleukin-3 (R&D systems, Minneapolis, MN), recombinant human interleukin-6 (R&D systems, Minneapolis, MN) and recombinant human stem cell factor (R&D systems, Minneapolis, MN) to a final concentration of 50 ng/ml each prior to intended use. .. N/A Bortezomib Sigma-Aldrich Cat#5043.14 Chloroquine Sigma-Aldrich Cat#PHR1258 Geldanamycin Sigma-Aldrich Cat#G3381 RIPA buffer Merck Millipore Cat#20-188 Complete Protease Inhibitor Roche Cat#05892970001 PhosSTOP phosphatase inhibitor Roche Cat#4906845001 Laemmli sample buffer Bio-Rad Cat#161-0737 b-mercaptoethanol Sigma-Aldrich Cat#M6250 4-12% bis-tris polyacrylamide gel BioRad Cat#4568093 PVDF membrane Carl Roth Cat# T830.1 BSA Carl Roth Cat#8076.4 Sure Block LuBio Science Cat#SB232010 ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat# P36962 A/G agarose beads Thermo Fisher Cat#20423 Trichloroacetic acid Sigma-Aldrich Cat#T6399 Acetone J.T.

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Concentration Assay:

    Article Title: Supramolecular assembly of GSK3α as a cellular response to amino acid starvation.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Asparaginase (pegaspargase) Shire Pharmaceuticals Oncaspar Trypan blue Invitrogen Cat#T10282 Human FLT3 R&D systems Cat#398-FK Human interleukin-7 R&D systems Cat#206-IL-010 Human stem cell factor R&D systems Cat#255-SC Puromycin Thermo Fisher Cat#A1113803 Neomycin Carl Roth Cat #CP11.2 Blasticidin Thermo Fisher Cat#R21001 Polybrene Merck Millipore Cat#TR-1003-G Lipofectamine 2000 Invitrogen Cat#11668030 Dexamethasone Sigma-Aldrich Cat#D4902 Vincristine Selleckchem Cat#S1241 6-Mercaptopurine Abcam Cat#ab142389 Rapamycin Selleckchem Cat#S1039 BRD5648 (Wagner et al., 2018) N/A BRD0705 (Wagner et al., 2018) N/A BRD3731 (Wagner et al., 2018) N/A HSP70 inhibitors B (Kang et al., 2014; Taldone et al., 2014). .. CD34+ progenitors were cultured in RPMI (Thermo Fisher Scientific) supplemented with 20% FBS and recombinant human interleukin-3 (R&D systems, Minneapolis, MN), recombinant human interleukin-6 (R&D systems, Minneapolis, MN) and recombinant human stem cell factor (R&D systems, Minneapolis, MN) to a final concentration of 50 ng/ml each prior to intended use. .. N/A Bortezomib Sigma-Aldrich Cat#5043.14 Chloroquine Sigma-Aldrich Cat#PHR1258 Geldanamycin Sigma-Aldrich Cat#G3381 RIPA buffer Merck Millipore Cat#20-188 Complete Protease Inhibitor Roche Cat#05892970001 PhosSTOP phosphatase inhibitor Roche Cat#4906845001 Laemmli sample buffer Bio-Rad Cat#161-0737 b-mercaptoethanol Sigma-Aldrich Cat#M6250 4-12% bis-tris polyacrylamide gel BioRad Cat#4568093 PVDF membrane Carl Roth Cat# T830.1 BSA Carl Roth Cat#8076.4 Sure Block LuBio Science Cat#SB232010 ProLong Diamond Antifade Mountant with DAPI Thermo Fisher Cat# P36962 A/G agarose beads Thermo Fisher Cat#20423 Trichloroacetic acid Sigma-Aldrich Cat#T6399 Acetone J.T.

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Centrifugation:

    Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome.
    Article Snippet: On days 0, 2, and 4 of differentiation, cells were cultured in SF- DIFF medium and further supplemented with the following cytokines and chemicals: day 0: 0.5 mM L- ascorbic acid (Sigma- Aldrich), 0.45 mM monothioglycerol (Fujifilm Wako Chemicals), 5 ng/mL recombinant human bone morphogenic protein- 4 (rhBMP- 4, R&D Systems, Minneapolis, MN, USA), 5 ng/mL recombinant human fibroblast growth factor 2 (rhFGF2, R&D Systems); day 2: 0.5 mM L- ascorbic acid, 0.45 mM monothioglycerol, 2 ng/ mL rhBMP- 4, 5 ng/mL rhFGF2, 5 ng/mL recombinant human vascular endothelial growth factor (rhVEGF, R&D Systems), and 2 ng/mL recombinant human/mouse/rat activin A (rhactivin A, R&D Systems); day 4: 0.5 mM L- ascorbic acid, 0.45 mM monothioglycerol, 25 μg/mL gentamicin (FUJIFILM Wako Pure Chemical Corporation), 5 ng/mL rhVEGF and 100 ng/ mL recombinant human colony stimulating factor 1 (rhCSF- 1, R&D Systems). .. On day 6, the culture medium was switched to StemPro- 34 medium (Thermo Fisher Scientific) containing 0.5 mM L- ascorbic acid, 0.45 mM monothioglycerol, penicillin–streptomycin (1×), 150 μg/mL transferrin from human serum (Roche, Basel, Switzerland), 2.0 mM glutamine, 50 ng/ mL rhCSF- 1, 100 ng/mL recombinant human stem cell factor (rhSCF, Nacalai Tesque), 10 ng/mL recombinant human interleukin- 3 (rhIL- 3, R&D Systems). mESCs or embryonic bodies were dissociated in TrypLE (1×, Thermo Fisher Scientific) for 4 min and collected by centrifugation at 200 × g for 5 min using a table- top centrifuge (Model2420, KUBOTA, Tokyo, Japan). .. For terminal differentiation towards primitive macrophages, collected cells were seeded with SF- DIFF (days 0, 2, and 4) or StemPro medium (day 6) into non- coated dishes (days 0, 2, and 6) and poly (2- hydroxyethyl methacrylate) (HEMA, SigmaAldrich)- coated plates (day 4) at 2.0–5.0 × 105 cells/mL and incubated under 5% CO2 at 37°C.

    Article Title: Preventing Differentiation Towards Primitive Macrophages in Stem Cells With Down Syndrome
    Article Snippet: On days 0, 2, and 4 of differentiation, cells were cultured in SF‐DIFF medium and further supplemented with the following cytokines and chemicals: day 0: 0.5 mM L‐ascorbic acid (Sigma‐Aldrich), 0.45 mM monothioglycerol (Fujifilm Wako Chemicals), 5 ng/mL recombinant human bone morphogenic protein‐4 (rhBMP‐4, R&D Systems, Minneapolis, MN, USA), 5 ng/mL recombinant human fibroblast growth factor 2 (rhFGF2, R&D Systems); day 2: 0.5 mM L‐ascorbic acid, 0.45 mM monothioglycerol, 2 ng/mL rhBMP‐4, 5 ng/mL rhFGF2, 5 ng/mL recombinant human vascular endothelial growth factor (rhVEGF, R&D Systems), and 2 ng/mL recombinant human/mouse/rat activin A (rhactivin A, R&D Systems); day 4: 0.5 mM L‐ascorbic acid, 0.45 mM monothioglycerol, 25 μg/mL gentamicin (FUJIFILM Wako Pure Chemical Corporation), 5 ng/mL rhVEGF and 100 ng/mL recombinant human colony stimulating factor 1 (rhCSF‐1, R&D Systems). .. On day 6, the culture medium was switched to StemPro‐34 medium (Thermo Fisher Scientific) containing 0.5 mM L‐ascorbic acid, 0.45 mM monothioglycerol, penicillin–streptomycin (1×), 150 μg/mL transferrin from human serum (Roche, Basel, Switzerland), 2.0 mM glutamine, 50 ng/mL rhCSF‐1, 100 ng/mL recombinant human stem cell factor (rhSCF, Nacalai Tesque), 10 ng/mL recombinant human interleukin‐3 (rhIL‐3, R&D Systems). mESCs or embryonic bodies were dissociated in TrypLE (1×, Thermo Fisher Scientific) for 4 min and collected by centrifugation at 200 × g for 5 min using a table‐top centrifuge (Model2420, KUBOTA, Tokyo, Japan). .. For terminal differentiation towards primitive macrophages, collected cells were seeded with SF‐DIFF (days 0, 2, and 4) or StemPro medium (day 6) into non‐coated dishes (days 0, 2, and 6) and poly (2‐hydroxyethyl methacrylate) (HEMA, Sigma‐Aldrich)‐coated plates (day 4) at 2.0–5.0 × 10 5 cells/mL and incubated under 5% CO 2 at 37°C.

    other:

    Article Title: Disruption of the tumour-associated EMP3 enhances erythroid proliferation and causes the MAM-negative phenotype
    Article Snippet: The annealed guide DNA oligonucleotides were then diluted 1:100 in cold 1× Tris-EDTA buffer (Sigma-Aldrich).

    Flow Cytometry:

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..

    Staining:

    Article Title: Concomitant inhibition of the thioredoxin system and nonhomologous DNA repair potently sensitizes Philadelphia‐positive lymphoid leukemia to tyrosine kinase inhibitors
    Article Snippet: For frozen samples whose viability after thawing was less than 90%, dead cells were discarded through centrifugation in the Lymphoprep (STEMCELL Technologies) gradient. .. Cells were seeded onto a 96‐well U‐bottom plate at 0.5 × 10 6 cells/mL in a final volume of 200 μL of SFEM II medium (Gibco), supplemented with 20% FBS (Gibco), 20 ng/mL recombinant human interleukin‐3 and 10 ng/mL recombinant human interleukin‐7 (R&D Systems), and cultured with drugs for 72 h. The percentage of viable cells was assessed by flow cytometry (LSRFortessa X‐20; BD Biosciences) after staining with propidium iodide (PI) at a final concentration of 1 μg/mL. ..



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